11 Core Difference between Single Stranded DNA and Double Stranded DNA

What is the difference between single-stranded DNA and double-stranded DNA?

Genetics is the study of inheritance. The genetic makeup of an organism is transmitted via DNA replication. The DNA can either be single-stranded or double-stranded.

The core difference between single-stranded DNA and double-stranded DNA is that single-stranded DNA has a star shape while double-stranded DNA has a linear form.

Difference between Single Stranded DNA and Double Stranded DNA

What Is Single-Stranded DNA?

Single-stranded DNA is a star-shaped genetic makeup and it is less stable. It behaves as randomly coiled polymers and it tends to be present in some viruses.

What Is Double-Stranded DNA?

Double-stranded DNA is a linear form of genetic makeup and it is quite stable. It behaves like a rigid rod-like structure and it is present in all organisms.

Comparison Chart: Single-Stranded DNA vs. Double-Stranded DNA

Basic Terms Single-Stranded DNADouble-Stranded DNA
AppearanceHave a stellate or star shapeHave a linear or filamentous shape
Composition RelationshipNo relationshipThymine is equal to adenine. Guanine is equal to cytosine
The ratio of A: T0.771
The ratio of G:C1.31
Stabilityless stableMore stable
Resistance to formaldehydeProne to actionQuite resistant
UV absorptionIncreases from 20 to 900cIncreases from 0 to 800c
MorphologyRandomly coiled polymerRigid rod-like structure
OccurrenceFew virusesAll organism
Chargaff ruleDo not followTend to follow
The ratio of purine and pyrimidineTend to vary1

Core Difference between Single-Stranded DNA and Double-Stranded DNA

  1. Single-stranded DNA occur in few viruses while double-stranded DNA occurs in all organism
  2. Double-stranded DNA follow chargaff rule while single-stranded DNA do not follow chargaff rule
  3. The ratio of purine and pyrimidine in single-stranded DNA tend to vary greatly while in double-stranded is one
  4. Thymine is equal to adenine in double-stranded DNA while there is no such relationship in single-stranded DNA
  5. Double-stranded DNA has linear or filamentous form while single-stranded has stellate or star shape form
  6. Single-stranded DNA have randomly coiled polymer while double-stranded DNA has a rigid rod-like structure
  7. The UV absorption of single-stranded DNA increases from 20 to 900c while that of double-stranded increases from 0 to 800c
  8. Double-stranded DNA is more stable while single-stranded DNA is less stable
  9. Double-stranded DNA is resistant to the action of formaldehyde while single-stranded is highly prone to the action of formaldehyde
  10. The ratio of guanine to cytosine in double-stranded DNA is one while in single-stranded DNA is 1.3
  11. The ratio of thymine to adenine in double-stranded DNA is one while in single-stranded is 0.77

Similarities between Single-Stranded DNA and Double-Stranded DNA

  1. Both deal with genetic material
  2. Both are polymers of nucleic acid
  3. Both contain purine and pyrimidine
  4. Both contain a nitrogenous base
  5. Both absorb UV light

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Comparison Video

Summary

The core difference between single-stranded DNA and double-stranded DNA is that single-stranded DNA has a stellate or star shape and double-stranded has rigid rod-like structures. Both deal with the genetic makeup of an organism.

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