20 Difference between Light Microscope and Electron Microscope (With Table)

Microscopes are available in different sizes and applications. It is the reason behind many learners finding it challenging to distinguish them. 

Light and electron microscopes are the most common microscopes in school laboratories. These instruments have unique features and meant for different purposes. 

Both microscopes use radiations to form a detailed image of an object that a human eye cannot see using naked eyes. These instruments are used at both the high school and university levels. 

So, what is the main difference between light microscope and electron microscope? The former is has a lower magnification while the latter has a higher magnification and resolution. 

This article provides detailed differences between light and electron microscope in a tabular form. Take the time to read through it and learn more about their similarities. 

Comparison Table (Light vs Electron Microscopes)

Basic TermsLight MicroscopeElectron Microscope
Year of Discovery1590 by Zoocharia Janssen1931 by Ernst Ruska and Max Knoll
Sources of illuminationVisible lightBeam of electrons
LensesCondenser lens, eyepiece, and objective lensProjector lens, objective, and condenser lens
Type of LensesGlass lens materialElectromagnetic lens material
Magnification Power1000X.            10,00,000X.
Resolving Power0.2 µm0.001 µm
Viewing ScreenImage is the view directly through the eyepieceImage is viewed on the fluorescent screen
Amount of PowerThe low power supply amountThe high power supply amount
Cooling systemNot availableReadily available
PreparationSimple and fast to prepare the sampleComplex to prepare the sample
TypesUltraviolet microscopy, bright field microscopy, confocal microscopy, darkfield microscopy, differential interference contrast microscopy, phase contrast microscope, and fluorescence microscopy.Scanning transmission electron microscope, scanning electron microscope, transmission electron microscope, focused ion beam, and electron microscope.
CostAffordableQuite expensive
Vacuum systemDoes not have a vacuum systemHave a vacuum system
FilamentDoes not have oneHave Tungsten filament
Leakage of RadiationNo leakageSusceptible to radiation  leakage
Colour of imageColouredBlack and white
Contrast imageSpecimen need to be stainedSpecimens need to be coated by heavy metal.
SizeComparatively smallerTend to be larger in size
Image VisibilitySuitable for both dead and living cellsOnly suitable for dead cells
Dimension of ImageGetting a detailed structure of the object is hard3D structure of the object
AvailabilityReadily availableNot available
Common Place of UsageIn Schools, Colleges and Universities LabsResearch extensive institutions and hospitals

What Is A Light Microscope?

A light microscope is also known as a compound microscope. The optical microscope uses sources of light to view a tiny object with transparent glasses and a projection screen.

The device is quite common in many labs of schools, colleges, and universities for viewing microorganisms. It is made of many parts that play different functions.

Some of the common parts of a light microscope are objective lenses. The eyepiece, diaphragm, light source, mirror, condenser, resolving nose piece, stage clips, tube, objective, coarse and fine focus.

The good news about the optical microscope is that it is simple and easy to handle. However, the resolution power tends to be low when compared to the electron microscope.

Here are some of the common types of compound microscope in the market right now: ultraviolet microscope, bright field microscopy, confocal microscopy, darkfield microscopy, differential interference contrast microscopy, phase contrast microscope, and fluorescence microscopy.

Advantages of a Light Microscope 

  1. Quite affordable 
  2. Suitable for living and dead specimens
  3. The natural color of objects is maintained 
  4. The image can be seen directly 
  5. No high electricity voltage is required 
  6. No effect of magnification 

Disadvantages of a Light Microscope 

  1. Revolving and magnification power is low 
  2. Preparation of specimen is tricky 
  3. Does not provide detailed information about an atom 
  4. Light beams do not travel in a straight path 
  5. Not ideal for small organisms 

What Is An Electron Microscope?

An electron microscope used electrons to view tiny objects instead of visible light as seen in a light microscope. The device is widely used by the scientist when to want to study finer details about an object.

The resolution power in an electron microscope is more advanced when compared to that of the light microscope. This is one of the reasons behind their popularity.

Some of the common types of electron microscope are scanning transmission electron microscopes, scanning electron microscope, transmission electron microscopes, focused ion beams, and electron microscope.

The electron microscope is meant to view details of an organelle due to its high power resolution property. The electrons have a short wavelength when compared to visible light.

Advantages of an Electron Microscope

  1. Has the best revolving power 
  2. The magnification is 10000 times 
  3. Provide a 3D image 
  4. Provide more clarity due to shorter wavelength 
  5. Provide detailed information about a specimen

Disadvantages of an Electron Microscope 

  1. Provide black and white images only 
  2. Tedious to prepare specimens 
  3. Quite complex to operate 
  4. Too expensive to acquire 
  5. Suitable for dead specimens
  6. Prone to leakages of radiations 

Difference between Light and Electron Microscopes

  1. The illuminating source in the light microscope is visible light while that of an electron microscope is a beam of electrons.
  2. The resolving and magnification power of a light microscope is 0.2 µm and 1000 times while that of an electron microscope is 0.001 µm and 10,000,000 times.
  3. Projection screen and glass lenses are used in light microscope while the fluorescent screen and an Electromagnetic screen are used in an electron microscope.
  4. The light microscope is able to obtain colored images of both living and dead cells while electron microscopes provide black and white color for dead cells only.
  5. The energy consumption in the electron microscope is quite high when compared to the light microscope.
  6. An electron microscope used tungsten filament which increases the risk of radiation leakage while a light microscope does not have a filament.
  7. The light microscope is simple to handle and easy to acquire while the electron microscope is not easy to operate and very expensive to acquire.
  8. An electron microscope is an extremely expensive instrument whereas a light microscope is quite affordable 
  9. The size of a light microscope is comparatively smaller while that of an electron microscope is comparatively larger
  10. Light microscope provide colored images whereas an electron microscope provides black and white images
  11. The natural color of objects can be visualized in a light microscope whereas cannot be visualized in an electron microscope
  12. Color imparting dyes are used in the light microscope for staining while the electron microscope uses heavy metals for staining
  13. The image can be observed by naked eyes under a light microscope whereas image cannot be observed by naked eyes under the electron microscope 
  14. Preparation of specimens for the light microscope is quite simple whereas the preparation of specimens for the electron microscope is tedious and complex
  15. The limited resolution of the light microscope is about 200 mm whereas that of the electron microscope is about 0.1 mm
  16. The objective and eyepiece lenses in the light microscope changes the magnification whereas the electric power or electric current changes the magnification of the electron microscope 
  17. Image formation under an electron microscope depends on the electron beams by different regions whereas that of the light microscope depend on differential absorption of light by different regions
  18. The specimen under the light microscope is mounted on a glass slide whereas that of an electron microscope is mounted  on a metallic grid
  19. Focusing under the light microscope is done by adjusting the lens mechanically whereas the electron microscope is done by adjusting the power of the electric current to electromagnetic lenses
  20. All the lenses of the light microscope are made of glass whereas those of the electron microscope are made of electromagnets. 

Similarities between Light and Electron Microscope

  1. Both are used for visualizing small objects that cannot be observed by eyes
  2. Both require specimen preparation by staining 
  3. Both are used in research application 
  4. Both can be used in microphotography
  5. Both follow Abbes law

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In Conclusion

The magnification of the electron microscope and the light microscope is what makes these two types of microscope stand apart.

However, they are quite essential for visualizing objects that cannot be seen by naked eyes. They are ideal for research applications.

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